Core Facility Information

The UB HIV and HCV Clinical Pharmacology Laboratory is an internationally recognized leader in antiviral pharmacology and therapeutics and has been conducting antiviral research since the beginning of the HIV epidemic. It was one of the initial National Institutes of Health Pharmacology Specialty Laboratories funded through the University of Rochester (UR) Clinical Trials Unit when the AIDS Clinical Trials Group (ACTG) was established. The laboratory is a training site for numerous national and international faculty, pre-doctoral students, post-doctoral fellows and residents. The international HIV and HCV clinical pharmacology research and training is supported by NIH funding for the NIAID HIV Clinical Pharmacology Quality Assurance Program, the NIAID AIDS Clinical Trials Group Pharmacology Specialty Laboratory and an HIV Training and Research Program (HTRP) in Clinical Pharmacology with the University of Zimbabwe from the Fogarty International Center.

The UB HIV and HCV Clinical Pharmacology Laboratory provides international and national leadership through IAS faculty membership; editorial board activity for www.Antimicrobe.org; Chair for the ACTG Clinical Pharmacology Laboratory Core; Membership on ACTG Transformative Sciences Group; Chair, Annual HIV Clinical Pharmacology Capacity Building Symposium at IAS and Co-Director, SUNY Global Health Institute.

As one of the leading antiviral pharmacology programs, we are focused in the following areas:

  1. Conducting innovative HIV and HCV clinical and translational research.
  2. Investigating novel biomatrices and compartments for the measurement of antiviral activity.
  3. Integrating pharmacokinetics and pharmacodynamics with human and viral genomics and proteomics to facilitate and accelerate drug development research.  
  4. Promote capacity building to train translational scientists and build global research infrastructure for antivirals and associated co-infections.

Our laboratory provides a global antiviral proficiency testing program through an NIH contract and conducts laboratory site assessments as a component of research quality assurance and capacity building (http://tdm.pharm.buffalo.edu/home/tprc/node/73).  We also are the PIs for the NIAID Precautionary and Prohibited Medications Database, a resource for NIH investigators designing protocols for antiviral drug development (http://tdm.pharm.buffalo.edu/home/tprc/node/70).

Chromatography Laboratory: The primary BSL-2 analytical space occupied by TPRC-dedicated staff and equipment within the current COE facilities is approximately 1,900 square feet and is equipped with back-up generator power, safety cabinets for chemical storage, instrument data ports, house air, deionized water, high purity liquid nitrogen, multiple -80°C storage units, biological safety cabinets and chemical fume hoods.  These areas support sample receipt, storage, processing and analysis. Samples are processed using solid-phase extraction or liquid-liquid extraction methods that are supported by an array of basic laboratory equipment.

Analytical equipment includes a fully equipped chromatography facility utilizing the newest ultra-performance liquid chromatography (UPLC) and traditional high performance liquid chromatography (HPLC) with state-of-the-art tandem mass spectrometry (MSMS), ultra-violet (UV), fluorescence and diode array detectors.

WATERS Chromatography Equipment

  • Pumps –1 model 515, 
  • Auto-injectors – 1 model 717 plus
  • Detectors:  - 3 Acquity TSQ Mass Spectrometer, 3 model Acquity photodiode array, 2 model 996 photodiode array, 1 model 2487 dual UV, 1 model 474 fluorescence, 1 model 2996 photodiode array, Separation Modules: 1 model Alliance 2695 with an Alliance column heater, 1 model Alliance 2795 with an Alliance column heater, 3 Aquity Ultra-performance Liquid Chromatography units (UPLCs), 1 Acquity Ultra-performance Liquid Chromatography (UPLC) H class.
  • Chromatography Software Manager:  Empower 2
  • Accessories:  two Waters temperature control modules with four banks of column heating blocks,  3 Powervar UPS model ABCDEF5200, 2 Dura-Cyl 230 liter nitrogen dewars with automatic manifold and station regulators and remote visual alarm.

 Freezers and Refrigerators:

  • Refrigerator-1 VWR
  • -20oC Freezers- 1 Jewett POLARSTAR, 2 Thermo Scientific Revco General Purpose Flammable Storage and Explosion proof
  • -70oC Freezers - 1 Harris, 6 Thermo Scientific Revco
  • Freezer Alarm System – Sensaphone 800 Remote Monitoring System

Genomics Laboratory: Equipment for genomics and molecular diagnosis includes 7300 real-time PCR system, Bio-Rad Western blot, a temperature-controlled plate reader for enzyme immunoassay, and regular PCR. All analytical systems are fully automated, networked and controlled by data processing software programs. All computers are networked for electronic download of results from the instrumentation into database specific study files.

Genomics Equipment:

  • 7300RT-PCR System
  • Thermal cycler TC-50A
  • Trans-Blot SD System and Powerpac HC Power supply

Hollow Fiber Membrane Pharmacodynamic/pharmacokinetic laboratory:

  • BSL-2 certified laboratory with back-up generator power, instrument data ports, house air, deionized water, bio-safety cabinet. Precision incubators, refrigerators and freezers, microcentrifuges, centrifuge, spectrophotometer, microscope, computerized syringe pump, computerized peristaltic pumps, Duet hollow fiber pump and magnetic heat/stir plates.

The TPRC laboratory operation has a peer quality assurance program that reviews all analytical procedures to comply with NYSDOH and CLIA requirements and operates closely with GLP standards. The TPRC laboratory’s analytical chromatographic equipment, electronic systems and software are covered under maintenance contract agreements. The TPRC laboratory utilizes the University’s Environment, Health & Safety department for the inspections of chemical fume hoods, and an outside company for biological safety cabinets and freezers, as well as other equipment not covered under warranties. 

The TPRC laboratory is staffed with highly trained and experienced scientific and support personnel, including NYSDOE-certified Medical Laboratory Technologists and Technicians. The TPRC staff are regularly trained in safe laboratory practices, including biological safety, chemical safety and chemical hazardous waste handling and disposal.

International Training Laboratory: The TPRC Laboratory facilities and resources include a dedicated International Training Laboratory to support research and training for international fellows. The International Training Laboratory features a dedicated ultra-performance liquid chromatography (UPLC) and traditional high performance liquid chromatography (HPLC) with state-of-the-art tandem mass spectrometry (MSMS) controlled by MassLynx Software, nitrogen generator, UPS, as well as general laboratory equipment, bench and office space (including desks, computers, telephones) and conference space for meetings, conferences, teleconferences and video conferences.